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It ought to be pointed out that due to the insufficient sensitivity of the USP pyrogen take a look at done on rabbits, "challenge" testing really should be conducted employing the Limulus Amebocyte Lysate Examination. Even though there isn't a manual With this spot, It could be envisioned that there be at least a three log reduction below the endotoxin challenge when the dilution method is used.
The commonest depyrogenation techniques for Bodily elements include incineration and removing by washing, also termed dilution. The literature has demonstrated other techniques, for example filtration, irradiation and ethylene oxide remedy to get constrained impact in decreasing pyrogen/endotoxin levels.
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There's been appreciable dialogue in the literature not too long ago pertaining on the Bacterial Endotoxins Exam, its importance and interpretation, and its comparison into the USP rabbit exam.
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